Question: Cell lineage identification from pathway anlaysis
0
gravatar for rob.costa1234
15 months ago by
rob.costa1234170
United States
rob.costa1234170 wrote:

From RNA seq data I am trying to find out the cell types enriched in particular cluster from DE genes, Is there any specific pathway or tool that can allow me to assign the cell identity using from DE genes. thanks

rna-seq • 616 views
ADD COMMENTlink modified 15 months ago by Kevin Blighe48k • written 15 months ago by rob.costa1234170
3
gravatar for Kevin Blighe
15 months ago by
Kevin Blighe48k
Kevin Blighe48k wrote:

What you are aiming to do is generally referred to 'cellular deconvolution'. Earlier methods were designed for microarray data and it is only now that methods for bulk and single cell RNA-seq are being developed.

It is suggested that one of the earlier efforts originally developed for microarray, i.e., CIBERSORT, can be used for RNA-seq data too. Other methods are in development. A worry that I have (for bulk RNA-seq) is that it is no longer the prime method for doing what you want to do. Single cell RNA-seq is superior in order to define cellular lineages, and methods are coming out more for single cell than they are for bulk RNA-seq, in that regard.

You may additionally want to look at the data available by tissue-specific consortia, such as:

What I suggest is that you obtain that data, identify the genes that define each tissue type, and then 'map' (deconvolute) your own data against those custom tissue signatures. Then you will have developed your own deconvolution method.

Kevin

ADD COMMENTlink modified 15 months ago • written 15 months ago by Kevin Blighe48k

CIBERSORT has one signature genes file LM22 for blood cells. However I want to identify stem cells, mesenchymal and fibroblasts. Is there any data base where I can get signature genes (at least name of genes) for different cell types?

Thanks

ADD REPLYlink written 15 months ago by rob.costa1234170

Those cell-types should be in the FANTOM5 dataset, however, you will have to find a custom way to overlay your data with the signatures from FANTOM5. I an give you some brief guidance, if you wan to first take a look there.

ADD REPLYlink written 15 months ago by Kevin Blighe48k

Kevin, Could you suggest any GUI/ web based old tool that has been used for cellular deconvolution of micro-array data? I googled it but could not find something.

ADD REPLYlink written 15 months ago by rob.costa1234170

Is CIBERSORT not okay for that purpose? You mention it in your other comment.

ADD REPLYlink written 15 months ago by Kevin Blighe48k

CIBERSORT need signature genes and provide only LM22 genes as signature for immune cells. It looks lot more complex to draw our own signature from http://fantom.gsc.riken.jp/data/.

Thus in the meantime I am just trying to see how the previous cellular deconvolution of micro-array data has evolved. But I dont see much information in this regard. I think if there is a web based database where if we plug in expression matrix and it identify possible cells will be a good starting point.

ADD REPLYlink written 15 months ago by rob.costa1234170

It hasn't been an area of intense research, put it that way, which is why there are not many options available. Another 'package' that I recall is CellMix, which allows you to use one of the most popular signatures, referred to as Abbas - this is for microarray but it is implemented in R.

I am sure that further methods will come out with the advent of GTEx and the Human Protein Atlas ( https://www.proteinatlas.org/tissue ).

You may want to take a look at this:

That lists some programs, and the final author of that is the guy who developed CellMix (I believe).

ADD REPLYlink written 15 months ago by Kevin Blighe48k
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