Hi, I am new to modeller software and please give me your advice on the followings. I modeled a protein by using the modeller software and obtained the templates for the target and in order to pick up suitable templates out of those, E-value is needed to consider.If E-value is lesser then the better the template according to their publication.But the E-values are in negative and do I have consider the value with the negetive or do I have to consider just the value?. I am confused with that.
and when selecting the templates do I have to consider both E-value and the sequence alignment between template and target because some of the templates are above 40% sequence identity and some of them are lesser than it?. and again when selecting a template based on the dendogram to generate the models based on it, do I have to consider both resolution and the sequence identity?