RPKM to TPM conversion?
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5.9 years ago

Hi all,

I have read past posts about the equations for these conversions and have come across these:

TPM(i) = ( FPKM(i) / sum ( FPKM  all transcripts ) ) * 10^6
TPM = (((mean transcript length in kilobases) x RPKM) / sum(RPKM all genes)) * 10^6

Can someone point me in a direction that validates these equations? Is there any scholarly articles or data sets with both TPM and RPKM where I can check these equations. I've been checking ncbi geo for a data set with both but can't seem to find one.

Thanks!

rpkm tpm conversion rna RNA-Seq • 11k views
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hi! Thank you, I just want to know if there is a way to validate these conversion equations / have other studies used this equation? I have no doubt that its "correct" I just want to ensure its valid before using it in my research

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hey... did you find any articles citing this equation or the validity of the TPM conversion? I am having the same issue

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Entering edit mode
5.9 years ago

Give a look to this vĂ­deo that explain the difference between RPKM, FPKM and TPM

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Entering edit mode
5.9 years ago

Also, Harold Pimmental's blog posting:

What the FPKM? A review of RNA-Seq expression units

Screen_Shot_2018_06_21_at_16_27_24

So, looks like you have it correct.

Kevin

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