Question: Polyester RNA-seq simulation
0
gravatar for k.kathirvel93
20 months ago by
k.kathirvel93220
India
k.kathirvel93220 wrote:

Hi EveryOne,

I am trying Polyester tool to simulate RNA-seq reads. i got a R code, but that will produce reads from chr22, but i want to generate reads from all chromosomes. I am very new to R. Can i get code or suggestions. Thanks in advance.

sequencing rna-seq next-gen • 526 views
ADD COMMENTlink modified 20 months ago by genomax78k • written 20 months ago by k.kathirvel93220

Without actual code you are using it is difficult for people to see what is going on. Please edit the original post and add your code/output samples.

ADD REPLYlink modified 20 months ago • written 20 months ago by genomax78k

Here is the github manual which i have used. Thank you

ADD REPLYlink modified 20 months ago • written 20 months ago by k.kathirvel93220
0
gravatar for genomax
20 months ago by
genomax78k
United States
genomax78k wrote:

The manual is only using sequence for chr22 as an example.

fasta_file = system.file('extdata', 'chr22.fa', package='polyester')

You will need to replace chr22.fa with a genome.fa, if you need the full genome.

ADD COMMENTlink written 20 months ago by genomax78k
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