Question: Polyester RNA-seq simulation
0
gravatar for k.kathirvel93
10 months ago by
k.kathirvel93190
India
k.kathirvel93190 wrote:

Hi EveryOne,

I am trying Polyester tool to simulate RNA-seq reads. i got a R code, but that will produce reads from chr22, but i want to generate reads from all chromosomes. I am very new to R. Can i get code or suggestions. Thanks in advance.

sequencing rna-seq next-gen • 303 views
ADD COMMENTlink modified 9 months ago by genomax65k • written 10 months ago by k.kathirvel93190

Without actual code you are using it is difficult for people to see what is going on. Please edit the original post and add your code/output samples.

ADD REPLYlink modified 10 months ago • written 10 months ago by genomax65k

Here is the github manual which i have used. Thank you

ADD REPLYlink modified 9 months ago • written 9 months ago by k.kathirvel93190
0
gravatar for genomax
9 months ago by
genomax65k
United States
genomax65k wrote:

The manual is only using sequence for chr22 as an example.

fasta_file = system.file('extdata', 'chr22.fa', package='polyester')

You will need to replace chr22.fa with a genome.fa, if you need the full genome.

ADD COMMENTlink written 9 months ago by genomax65k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1097 users visited in the last hour