Websites to asses my primers
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3.3 years ago
apl00028 ▴ 70

I have designed some primers with some degenerated nucleotide which I would like calculate the tm, and the probability of hairpin and self dimer. So far, I have used OligoAnalyzer website but today is out. I would like calculate them with another alternative webiste. Any suggestion?

I'd appreciate any piece of information on this issue, thanks!!!!

primer sequence • 713 views
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Entering edit mode
3.3 years ago
ATpoint 54k

PrimerThree

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