I have paired-end Illumina Files named
The 7_2 file being the forward and 7_1 being the reverse.
The name of the Trinity Output Assembly file is
the paths to these files are:
~/Desktop/Mantle_TT_7_2_TGACCAqtrim.fq ~/Desktop/Mantle_TT_7_1_TGACCAqtrim.fq ~/Desktop/TrinityMantleTT.fasta
I'm trying to run this with the following command:
perl rsem-eval-calculate-score --paired-end ~/Desktop/Mantle_TT_7_2_TGACCAqtrim.fq \ ~/Desktop/Mantle_TT_7_1_TGACCAqtrim.fq \ ~/Desktop/TrinityMantleTT.fasta Mantle_TT_rsem_eval
But I keep getting an error saying
invalid number of argumentss
Am I listing my paired-end files incorrectly? Am I missing something?