Question: What is wrong with NCBI's gff file?
gravatar for rimgubaev
14 months ago by
rimgubaev140 wrote:

This is a part of gff file for Nicotiana tabacum (tobacco) genome fasta. I must say that the fact that mRNA, gene, 1st exon and 1st CDS start at the same location (2309) is quite confusing! Any ideas?

NW_015787321.1  Gnomon  gene    2309    5631    .   +   .   ID=gene43;Dbxref=GeneID:107775605;Name=LOC107775605;gbkey=Gene;gene=LOC107775605;gene_biotype=protein_coding
NW_015787321.1  Gnomon  mRNA    2309    5631    .   +   .   ID=rna56;Parent=gene43;Dbxref=GeneID:107775605,Genbank:XM_016595671.1;Name=XM_016595671.1;gbkey=mRNA;gene=LOC107775605
NW_015787321.1  Gnomon  exon    2309    3923    .   +   .   ID=id448;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XM_016595671.1;gbkey=mRNA;gene=LOC107775605
NW_015787321.1  Gnomon  exon    3967    5133    .   +   .   ID=id449;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XM_016595671.1;gbkey=mRNA;gene=LOC107775605
NW_015787321.1  Gnomon  exon    5273    5631    .   +   .   ID=id450;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XM_016595671.1;gbkey=mRNA;gene=LOC107775605
NW_015787321.1  Gnomon  CDS 2309    3923    .   +   0   ID=cds47;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XP_016451157.1;Name=XP_016451157.1;gbkey=CDS;gene=LOC107775605
NW_015787321.1  Gnomon  CDS 3967    5133    .   +   2   ID=cds47;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XP_016451157.1;Name=XP_016451157.1;gbkey=CDS;gene=LOC107775605
NW_015787321.1  Gnomon  CDS 5273    5631    .   +   2   ID=cds47;Parent=rna56;Dbxref=GeneID:107775605,Genbank:XP_016451157.1;Name=XP_016451157.1;gbkey=CDS;gene=LOC107775605
gff • 590 views
ADD COMMENTlink modified 14 months ago • written 14 months ago by rimgubaev140

I'd recommend you add (to your post by editing it) the common name of the organism as well as why you think what you're seeing is weird.

ADD REPLYlink modified 14 months ago • written 14 months ago by RamRS24k
gravatar for Damian Kao
14 months ago by
Damian Kao15k
Damian Kao15k wrote:

Gene, mRNA and Exon starting with the same coordinate is normal. The CDS features starting at the same coordinate suggest that the 5'UTR wasn't annotated for this gene. This is probably due to this gene being computationally predicted rather than annotated with biological evidence (Ie. RNA-seq).

ADD COMMENTlink modified 14 months ago • written 14 months ago by Damian Kao15k

Now it's clear, thanks!

ADD REPLYlink written 14 months ago by rimgubaev140
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