Question: Should --very-sensitive be combined with -no-1mm-upfront?
gravatar for Lucy
13 months ago by
Lucy20 wrote:


I am planning to use the --very-sensitive option for aligning my ATAC-seq reads. However, after reading the manual section below, I am unclear as to whether this option should be combined with --no-1mm-upfront or not?

"By default, Bowtie 2 will attempt to find either an exact or a 1-mismatch end-to-end alignment for the read before trying the multiseed heuristic. Such alignments can be found very quickly, and many short read alignments have exact or near-exact end-to-end alignments. However, this can lead to unexpected alignments when the user also sets options governing the multiseed heuristic, like -L and -N."

Many thanks,


bowtie bowtie2 • 269 views
ADD COMMENTlink modified 13 months ago by Devon Ryan92k • written 13 months ago by Lucy20
gravatar for Devon Ryan
13 months ago by
Devon Ryan92k
Freiburg, Germany
Devon Ryan92k wrote:

--very-sensitive doesn't change the permitted number of mismatches to 0, so you don't need to specify --no-1mm-upfront.

ADD COMMENTlink written 13 months ago by Devon Ryan92k
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