Question: R program for normalization of RNA seq counts data with Edge R or Deseq
0
gravatar for maryak
2.3 years ago by
maryak20
maryak20 wrote:

Can anybody guide me about normalization of RNA seq( Htseq-counts data ) using edgeR or Deseq2,i need help about R program for normalization of RNA seq using EdgeR.

sequencing rna-seq R • 3.2k views
ADD COMMENTlink written 2.3 years ago by maryak20
2
gravatar for GenoMax
2.3 years ago by
GenoMax94k
United States
GenoMax94k wrote:

Normalization of the data is managed by the packages mentioned. You need to feed them raw count data.

ADD COMMENTlink written 2.3 years ago by GenoMax94k

problem is i don't know the exact way of using these packages and feeding data to them

ADD REPLYlink written 2.3 years ago by maryak20
2

Workflow for DESeq2 is described in detail here. For edgeR you can find similar information here.

ADD REPLYlink written 2.3 years ago by GenoMax94k
1

In addition, focus on the section in the DESeq2 manual, htseq-count input.

ADD REPLYlink written 2.3 years ago by ATpoint44k

i have ht-seq-count input in the form of excel file(CSV) but in these link i am not able to find how to normalize such kind of files..

ADD REPLYlink written 2.3 years ago by maryak20
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1511 users visited in the last hour
_