R program for normalization of RNA seq counts data with Edge R or Deseq

Question

[maryak]

Can anybody guide me about normalization of RNA seq( Htseq-counts data ) using edgeR or Deseq2,i need help about R program for normalization of RNA seq using EdgeR.

Answer 1

[GenoMax]

Normalization of the data is managed by the packages mentioned. You need to feed them raw count data.

[maryak]

problem is i don't know the exact way of using these packages and feeding data to them

[GenoMax]

Workflow for DESeq2 is described in detail here. For edgeR you can find similar information here.

[ATpoint]

In addition, focus on the section in the DESeq2 manual, htseq-count input.

[maryak]

i have ht-seq-count input in the form of excel file(CSV) but in these link i am not able to find how to normalize such kind of files..