Question: R program for normalization of RNA seq counts data with Edge R or Deseq
0
gravatar for maryak
8 months ago by
maryak10
maryak10 wrote:

Can anybody guide me about normalization of RNA seq( Htseq-counts data ) using edgeR or Deseq2,i need help about R program for normalization of RNA seq using EdgeR.

sequencing rna-seq R • 933 views
ADD COMMENTlink written 8 months ago by maryak10
2
gravatar for genomax
8 months ago by
genomax67k
United States
genomax67k wrote:

Normalization of the data is managed by the packages mentioned. You need to feed them raw count data.

ADD COMMENTlink written 8 months ago by genomax67k

problem is i don't know the exact way of using these packages and feeding data to them

ADD REPLYlink written 8 months ago by maryak10
2

Workflow for DESeq2 is described in detail here. For edgeR you can find similar information here.

ADD REPLYlink written 8 months ago by genomax67k
1

In addition, focus on the section in the DESeq2 manual, htseq-count input.

ADD REPLYlink written 8 months ago by ATpoint17k

i have ht-seq-count input in the form of excel file(CSV) but in these link i am not able to find how to normalize such kind of files..

ADD REPLYlink written 8 months ago by maryak10
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