FASTA to Gene Names (Is BLAST the best option)
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5.7 years ago
gtasource ▴ 60

Dealing with an unannotated genome. I have the sequences of genes I'm interested in knowing the names of (in fasta format.) The idea was to simply BLAST them against a species that we believe is closely related. However, is there any other options or software that might be best suited?

BLAST • 888 views
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Is this a pro- or eukaryotic genome. With prokaryotic genomes prokka (https://github.com/tseemann/prokka ) is what people recommend for annotation of new genomes.

You could simply blast (do it at protein level to get better matches) against a closely related genome if one is available.

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Blasting will not give the names of the genes, it will give you the names of the most similar genes in a database. Have in mind that the names of those genes in the database may have been themselves derived from blast searches.

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