Question: (Closed) Python Script to Calculate Total Number of genes
0
gravatar for anasjamshed1994
4 weeks ago by
anasjamshed19940 wrote:

This is the HCV Genome Sequence:

>GU294484.1 Hepatitis C virus isolate PK-1, complete genome
ACCTGCCTCTTACGAGGCGACACTCCACCATGGATCACTCCCCTGTGAGGAACTACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTATGAGTGTCGTGCAGCCTCCAGGACCCCCCCTCCCGGGAGAGCCATAGTGGTCTGCGGAACCGGTGAGTACACCGGAATTGCCAGGACGACCGGGTCCTTTCTTGGATAAACCCGCTCAATGCCTGGAGATTTGGGCGTGCCCCCGCAAGACTGCTAGCCGAGTAGTGTTGGGTCGCGAAAGGCCTTGTGGTACTGCCTGATAGGGTGCTTGCGAGTGCCCCGGGAGGTCTCGTAGACCGTGCAACATGAGCACACTTCCTAAACCTCAAAGAAAAACCAAAAGAAAACCCATCCGTCGCCCACAGGACGTCAAGTTCCCGGGTGGCGGACAGATCGTTGGTGGAGTATACGTGTTGCCGCGCAGGGGCCCACGATTGGGTGTGCGCGCGACGCGTAAGGCTTCTGAACGGTCACAGCCTCGCGAACGACGACAGCCTATCCCCAAGGCGCGTCGGAGCGAAGGCCGGTCCTGGGCTCAGCCTGGGTACCCTTGGCCCCTCTATGGTAATGAGGGCTGCGGGTGGGCAGGGTGGCTCCTGTCCCCCCGCGGCTCCCGTCCATCTTGGGGCCCAAACGACCCCCGGCGAAGATCCCGCAACTTGGGTAAAGTCATCGATACCCTTACGTGCGGATTCGCCGACCTCATGGGGTACATCCCGCTCGTCGGCGCTCCCGTAGGAGGCGTCGCAAGAGCCCTCGCGCATGGCGTGAGGGCCCTTGAAGACGGGATAAATTTTGCGACAGGGAACTTGCCCGGTTGCTCCTTTTCTATCTTCCTTCTTGCTCTACTCTCTTGCTTAATTCATCCAGCAGCCAGTCTAGAGTGGCGGAATACGTCTGGTCTCTATGTCCTTACCAACGCCCGTTCCAACAGCAGTATAGTGTACGAGGCCGACGACGTTATCCTGCACACACCCGGCTGTATACCTTGTGTTCAGACCGGCAACACATCCAAGTGCTGGACCCCAATGACACCCACGGTGGCAGTTAAGTATGTCGGAGCAACCACCGCTTCGATACGCGGTCATGTGGACCTGTTAGTGGGCGCAGCCACGATGTGTTCTGCGCTCTACGTGGGTGATGTGTGCGGAGCCGTCTTCCTCGTGGGGCAAGCCTTCACGTTCAGGCCGCGACGCCATCAAACGGTCCAGACCTGCAACTGCTCGCTGTACCCAGGCCATCTCACAGGACATCGAATGGCTTGGGATATGATGATGAACTGGTCCCCTGCTGTTGGCATGGTGGTGGCGCACATCTTACGCCTACCCCAGACCCTGTTTGATATAATAGCCGGGGCCCATTGGGGCGTCTTGGCGGGTCTAGCCTACTATACCATGCAGGGCAACTGGGCCAAGGTCGCAATCATCATGGTTATGTTCTCAGGGGTCGATGCCGTTACGTACATCACTGGGGGCACTGCAGCTCGTGGGGGCCAAGGGCTGGCTAGCCTAATCGTCCGGGGGCCTGAGCAGCGCCTGGAGCTGATCAACACCCATGGCTCGTGGCACATCAACAGTACTGTCCTCCACTGCAATGAGTCCATAAACACAGGGTTTATAGCTGGGTTGTTTTATTATCATAAGTTCAACTTACTGGATGTCCCGAAGGCTCAGCAGCTGCAAGCCCATCACTTTCTTCAGGCAGGGGTGGGGCCCCTTGACAGATGCCAACATCCACCGGCCCTTCTGATGACAACCGTACTGCTGGCATACGCACCTAGACCTTGTGACAGCGTAAAGCAGCACGTGTCTCCGGTCCTGTGTATGCTTCCACACCATCGCCCAGTGGTGGTAGGCACTACTGATCCTAAGGGCGCTCCCACCTATAACTGGGGCGAGAATGAGACAGACGTGTTCCTGCTGAATCCCTGCGGCCTCCTAGTGGTCGGTGGTTTGGGTGGCACGTGGGAGGAACTCCACCGGGGTTTGTCAAGACGTGCGGAGGTTCCCCCTTGTGACATCTATGGGGGTGGGGGGGAGATCCACCAATGGTTCAGACCTCTTCTGCCCCACCGACTGCTTCAGGAAACATCCCGAGGCCACATACAGCCGGTGCGGCTCGGGGCCCTGGTTGACACCTCGATGCATGGTCGACTATCCATACCGGCTTTGGCATTACCCATGTACAGTCAATTTTACACTGTTCAAGGTGAGGATGTTTGTGGGTGGGTTTGGCATCGGTTTACCGCCGCTTGCAACTGGACTAGGGGGGAGCGCTGCGATATCGAGGATCGTGACCGCAGCGAGCAACATCCCCTGCTGCATTCAACAACTGAGCTTGCCATACTGCCTTGCTCTTTCACGCCCATGCCCGCATTGTCAACAGGGTTAATACACCTCCACCAAAACATCGTGGATGTCCAATACCTTTATGGCGTTGGATCTGGCATGGTGGGATGGGCGTTGAAATGGGAATTTGTCATCCTCGTTTTCCTCCTCCTAGCAGATGCACGCGTGTGCGTTGCCCTTTGGCTGATGCTGATGATATCACAAGCAGAAGCAGCCTGGAGAACTTGTCACGCTGAACGCCGTCTCTGCTGCCGGGACACATGGTATCGGCTGGTACCTGGTAGCATTTTGCGCGGCGTGGTACGTGCGGGGAAACTCGTCCCGCTGGTGACCTACAGCCTGACGGGTCTTTGGTCCCTAGCATTGCTCGTCCTTCTACTCCCCCAGCGGGCGTATGCTTGGTCGGGTGAAGACAGTGCCACCCTCGGCGCTGGGATCTTGGTCCTCTTCGGCTTCTTTACCCTGTCACCTTGGTATAAGCACTGGATCAGCCGCCTCATGTGGTGGAACCAGTACGCCATATGTAGGTGTGAGTCTGCTCTCCAAGTATGGGTCCCCCCCCTACTTGCCCGCGGGAGTAGGGACGGTGTTATCCTGCTAACAAGCCTGCTTTATCCATCATTAGTTTTTGACATCGCTAAGCTGCTGATAGCCGTAATAGGCCCATTATATCTAATACAGGCCGCCATCACTACTACCCCCTACTTTGTGCGTGCGCATGTTCTGGTCCGCCTTTGCATGTTCGTGCGCTCCGTGACGGGGGGAAAGTACTTCCAGATGGCCATACTGAGCGTCGGCAGATGGTTTAACACCTACCTATATGACCACCTTGCACCGATGCAACACTGGGCCGCAGCAGGCCTCAAAGACCTGGCAGTAGCCACTGAACCTGTAATATTCAGTCCCATGGAAATCAAGGTCATCACTTGGGGCGCGGACACGGCAGCTTGCGGAGATATCCTATGCGGGCTGCCCGTCTCTGCACGATTAGGCCGTGAGGTGTTGTTGGGACCTGCTGATGACTATCGGGAGATGGGCTGGCGTCTGTTGGCCCCGATTACAGCATACGCCCAGCAAACTAGGCGTCTTTTTGGGACTATTGTGACCAGCTTGACTGGCAGGGACAAGAACGTGGTGGCCGGCGAAGTGCAGGTGCTTTCTACGGCTACCCAGACCTTCCTAGGTACAACATTGGGAGGGGTTATGTGGACTGTTTACCATGGAGCAGGTTCGAGAACACTTGCGGGCGTCAAACATCCTGCGCTCCAAATGTACACAAATGTAGATCAGGACCTCGTTGGATGGCCAGCTCCTCCGGGGGCTAAGTCTCTTGAACCGTGCACCTGCGGGTCTGCGGACTTGTACTTGGTTACCCGCGAAGCTGATGTCATCCCTGCTAGACGCAGGGGGGACTCCACAGCGAGCTTGCTCAGTCCTAGGCCTCTCGCCTGTCTCAAAGGTTCCTCTGGAGGTCCTGTTATGTGCCCTTCGGGCCACGTAGCGGGGATCTTTAGGGCTGCTGTGTGCACCAGAGGTGTAGCAAAAGCCCTACAGTTCATACCAGTGGAAACCCTTAGCACACAGGCTAGGTCTCCATCCTTTTCTGACAATTCAACTCCTCCTGCTGTTCCACAGAGCTATCAAGTAGGGTACCTTCATGCCCCGACCGGCAGCGGTAAGAGCACAAAGGTCCCGGCCGCTTATGTAGCACAAGGATATAATGTTCTCGTGTTGAATCCATCAGTGGCGGCCACACTAGGCTTCGGCTCTTTCATGTCGCGAGCTTATGGGATCGACCCCAACATCCGCACCGGGAACGGCACGGTTACAACTGGTGCTAATCTGACCTATTCCACCTATGGTAAGTTTCTCGCGGACGGGGGTTGCTCGGGGGGAGCATATGATGTGATTATCTGTGATGAGTGTCATGCCCAAGACTCTACTAGCATACTGGGTATAGGCACGGTCCTAGATCAGGCTGAAACGGCTGGGGTGAGGCTGACGGTTTTAGCAACAGCAACTCCCCCAGGCAGCATCATTGGGCCCCATTCTAACCTCAAAGAAGTGGCCCTTGGTTCTGAGGGGGAGATCCCTTTCTTCGGCAAGGCCATACCGCTAGCCCTGCTAAGGGGGAAAGGCACCTTATTTTTTTCCATTCCAAGAAAAAATGTGATGAGATGGCATCCAAACTCAGAGGCATGGGGCTCAACGCTGAAGGAGTACTACAGGGGTCTTGATGTGTCCGTCATACCAACATCAGGAGACGTTGTAGTTTGCGCTACTGACGCCCTCATGACTGGATTCACCGGAGACTTCGACTCTGTCATAGATTGCAACGTGGCTGTTGAACAGTACGTTGATTTCAGCTTGGACCCCACCTTTTCCATTGAGACTCGCACTGCTCCCCAAGACGCGGTTTCCCGCAGTCAACGTCGTGGCCGTACGGGCCGAGGTAGACTCGGCACGTACCGATATGTCACCCCCGGTGAAAGACCGTCTGGGATGTTTGACTCGGCTGTTCTCTGTGAGTGCTATGACGCGGGCTGCTCGTGGTACGACTTGCAGCCCGCCGAGACCACAGTCAGACTAAGAGCTTACTTGTCCACGCCGGGGTTACCTGTCTGCCAAGACCACTTGGAATTTTGGGAGAGCGTCTTCACTGGACTAACTCACATAGATGCCCACTTTCTATCACAGACCAAGCAGCAGGGACTCAACTTCCCATACCTAGCTGCCTACCAAGCCACTGTGTGCGCTCGCGCGCAAGCTCCTCCCCCAAGTTGGGACGAGACATGGAAGTGTCTCGTGCGGCTTAAGCCAACACTACATGGACCTACACCCCTTCGATATCGGCCGGGGCCTGTCCAAAATGAAACCTGCTTGACACACCCCATCACAAAATACCTCATGGCATGCATGTCAGCCGATCTGGAAGTAACCACCAGCACCTGGAGCACCTGGGTGTTGCTCGGAGGGGTCCTCGCGGCCCTGGCAGCCTACTGCTTGTCGGTCGGCTGCGTAGTCATTGTGGGCCACATTGAGCTGGGGGGCAAGCCGGCGCTCGTTCCTGACAAAGAAGTGTTGTATCAACAATACGATGAGATGGAGGAGTGCTCACAAGCTGCCCCATATATCGAACAAGCTCAAGTAATAGCCCACCAGTTCAAGGAAAAAGTCCTTGGATTGCTACAGCGAGCTACCCAACAACAAGCTGTCATTGAGCCCATAGTAGTTACCAACTGGCAAAAGCTTGAGGCCTTCTGGCACAAGCACATGTGGAACTTTGTGAGTGGGATTCAGTACCTAGCAGGTCTCTCCACTTTGCCCGGCAACCCCGCTGTGGCGTCTCTTATGGCGTTCGCTGCTTCAGTCACCAGTCCCCTGACGACCAATCAAACTATGTTTTTTAACATACTCGGGGGATGGGTTGCTACTCATTTGGCAGGGCCCCAGAGCTCTTCCGCATTCGTGGTAAGCGGCTTGGCCGGCGCTGCCATAGGGGGCATAGGCCTGGGCAGGGTCTTACTTGACATCCTGGCAGGATACGGAGCTGGTGTCTCAGGCGCCTTGGTGGCTTTCAAAATCATGGGGGGGGAACTCCCCAATGCCGAGGACGTGGTCAATCTGTTGCCCGCCATACTATCTCCGGGTGCTCTCGTCGTCGGGGTGATATGCGCTGCCCTACTACGTCGGCACGTGGGACCTGGGGAGGGAGCGGTACAGTGGATGAACAGGCTCATCGCGTTCGCATCCCGGGGCAACCACGTCTCACCGACGCACTATGTTCCCGAGAGCGATGCTGCGGCAAGGGTCACCGCATTGCTGAGTTCTCTAACTGTCACAAGTCTGCTCCGGCGGTTACACCAGTGGATCAATGAAGACTACCCAAGCCCTTGTAGCGACGATTGGCTACGTACCATCTGGGACTGGGTCTGCATGGTGTTGCTCGACTTCAAGACATGGCTGTCTGCTAAGATCATGCCATTGCTCCCTGGGTTGCCCTTCATTTCCTGTCAAAAGGGATATAAGGGCGTTTGGCAGGGGGACGGCGTGGTGTCCACTCGCTGTCCTTGCGGAGCAGTGATAACCGGTCATGTGAAGAACGGGTCCATGCGGCTTGCAGGACCACGTACATGTGCTAACATGTGGCACGGCACCTTCCCCATCAACGAGTACACCACCGGACCCAGCACACCTTGCCCATCACCCAACTACACTCGTGCACTGTGGCGCGTGGCTGCCAACAGCTACGTCGAAGTGCGACGGGTGGGAGACTTCCACTACATCACGGGGGCCACAGAAGATGAGCTCAAGTGTCCGTGCCAAGTGCCGGCTGCTGAGTTCTTTACTGAAGTGGATGGGGTGAGACTTCACCGTTACGCCCCTCCATGCAGGCCCCTGTTGAGGGATGAGATCACTTTCGTAGTAGGGCTGAATTCTTACGCGATAGGATCCCAACTCCCTTGTGAGCCCGAACCGGACGTCTCTGTGCTGACCTCGATGTTGAGAGACCCTTCCCATATCACCGCCGAGACGGCAGCGCGCCGCCTTGCACGCGGGTCCCCTCCATCAGAGGCAAGCTCATCCGCCAGTCAACTATCGGCTCCATCGTTGAAGGCCACTTGCCAAACGCATAGGCCTCATCCCGACGCGAGCTGGTGGACGCCAACTTGTGTTTGGCGACAAGAGATGGGCAGCAACATCACACGGGTAGAGTCCGAAACAAAGGTTGTGATTCTTGACTCATTCGAACCTCTGAGGGCCGAGACTGATGACACCGAGCTCTCGGTAGCAGCAGAGTGTTTCAAGAAACCTCCCAAGTATCCTCCAGCCCTCCCTATCTGGGCTAGGCCAGACTACAACCCTCCACTGTTGGATCGTTGGAAATCACCGGATTATGAACCACCAATTGTTCATGGGTGCGCCTTACCACCACAGGGTACTCCACCGGTGCCTCCCCCTCGGAGGAAAAGAACAATCCAGCTGGACGGCTCCAATGTGTCCGCGGCGCTAGCTGCGCTAGCGGAAAAATCATTCCCGGCCTCAAAACCGTTGGAAGCGGGTAGCTCATCCTCAGGGGTCGATACACAGTCCAGCACTACTTCCAAGGTGCCTCCCTCTTCGGAGAGAGAGTCCGACACAGAATCGTGCTCGTCCATGCCTCCTCTCGAGGGGGAGCCGGGCGATCCAGACTTGAGTTGCGACTCTTGGTCCACTGTTAGTGACAGCGAGGAGCAGAGCGTGGTCTGCTGCTCTATGTCGTATTCTTGGACCGACGCCCTGATAACACCATGTAGTGCTGAGGGAGAGAACTGCCCATCAGCCCACTCAGCAATCTTGGAGAGACATCACAACCTAATCTATTCAACGTCGTCTAGAATCGCTTCTCAACGTCAGAAGAAGGTCACCTTCGACAGGCTGCAGGTGCTCGACGACCATTACAAAACTGCATTAAAGGAGATAAAGGAGCGAGCGTCAAGGGTAAAGGCTCGCATGCTCACCATCGAGGAAGCGTGCGCGCTCGTCCCTCCTCACTCTGCTCGGTCAAAGTTCGGGTATAGTGCGAAGGACGCTCGCTCCCTGTCCAGCAAGGCCATTAACCAGATCCGCTCCGTCTGGGAGGACTTGCTGGAAGACACCACAACTCCAATTCCAACCACCATCATGGCGAAGAGCGAGGTTTTTTGTGTGGATCCTACTAAAGGAGGCCGTTTTTTGCTCGTCTCATTGTCTACCCTGACCTGGGGGTGCGCATCTGTGAGAACGTGCCCTATATGGCGTGATACAGAAGTGGGGAGTGGGGACGATGGGTCCTGCCTATGGATTCCAATACTCGCCTCAACAGCGGGTCGAACGTCTGCTGAAGATGTGGACCTCAAAGAAAGCCCAGTTGGGGTTCTCGTATGGTACCCGCTGCTTTGGCTCGACTGCCACTGGACAGGACATCAGGGTGGAAGAGGAGATATACCAATGCTGGAGCCTTGGACCGGAGGCCAGGAAAGTGATCTCCTCCCTCACGGAGCGGCTTTACTGCGGAGGCCCTATGTTCAACAGCAAGGGGGCCCAGTGTGGTTATCGCCGTTGCCGTGCCAGTGGAGTTCTGCCTACCAGCTTCGGCAACACGATCACTTGTTACATCAAGGCCACAGCGGCTGCAAAGGCCGCAAACCTCCGGAAGCCTGGCTTCTTGTTTGGGGAGGATGGATCCTGGTCGTATTACCTGAGGACCGAATGGGGTTCGAATGGAGATACGGGCAGTCCTGGGAGAGCCTTCACCGGAGGCTATGGACCAGGTATTTCTTGCTTCCACCCGGAGATGGCCCCCACAGGCCAACCCTACGACCTTTGGGCTCATTACATCTGGCTCCTCCAACGTCTCCGTGGCGCGGGACGATACGGGGAAGAGGTATTATTACCTCACTCGTGGTGCCACCACCCCCCTGGCCCGTGCTGCTTGGGAGACAGCTCGTCACACTCCAGTTACCTCCTGGCTGGGGAACATCATCATGTACGCGCCTACTATTTGGGTGCGCATGGTGGTGGTGGCACACTTTTTCTCCATACTCCAATCCCAGGAGATACTTGGTCGCCCCCTTGGCTTTGGAATGTACGGGGCCACTTACTCTGTCACTCCGCTGGATTTACCAGCAATCATTGGAAGACTCCATGGTCTACGCGCGTTTACGCTCCATATTTACTCTCCAGCAGAGCTCAATACGGTCGCGGGGACACTCAGGAAGCTTGTGCCCCCCCCTACGAGCTTGGAGACATCGGGCACGAGCAGTGCGCGCTATGCTTATCGCCCAGGGAGGGAAGGCCAGGATTTGTGGGCTTTATCACTTCAATTGGGCGGTACGCACCAAGACCACCCTCACTCCACTGCCAGCCGCTGGCCAGTTGGATTTATCCATCTGGTTTACGGTTGGTGTCGGCGGGAACGACATTCTCGCAGCGTGTCACGCGCCCGAACCCGCCATTTGCTGCTTTGCCTACTCCCTACTAACAGTAGGGGTAGGCATCTTTCTCTTGCCAGCTCGATGAGCTGGTAAGATAACACTCCATTTCTTTTTTGTTTTTTTTTTTTTTTTTTTT

I want to write Python script to Calculate Total Genes present in this 9474 bp sequence. The Start Codon is (ATG) and i want to calculate the genes from all 3 reading frames

ADD COMMENTlink modified 23 days ago • written 4 weeks ago by anasjamshed19940
2

Is this just a 'toy' exercise? If so, this might get you started:

https://rody.blog/2015/11/14/orf-finder-for-fasta-files-with-dna-sequences-using-python/

If not, you should know that gene finding is more subtle than simply finding all ATG...STOP sequences.

ADD REPLYlink modified 4 weeks ago by Istvan Albert ♦♦ 78k • written 4 weeks ago by jrj.healey8.6k

i want to calculate total number of genes present in my sequence not ORF

ADD REPLYlink written 29 days ago by anasjamshed19940
1

Sounds like a school project, could you show what you have tried to get a starting point to work on ?

ADD REPLYlink written 4 weeks ago by Bastien Hervé2.3k
>>> from Bio.Seq import Seq
>>> genome = open("C:\\Users\\cnn\\Desktop\\MS(BI)\\HCV.txt")                     #( Open .txt file)
>>> print(genome)
 >>> Data=genome.read()                                                    #( Read .txt file)
>>> print (Data)
ADD REPLYlink modified 28 days ago by jrj.healey8.6k • written 4 weeks ago by anasjamshed19940

That is not how you utilise BioPython. Take a look at the link I gave you.

ADD REPLYlink written 4 weeks ago by jrj.healey8.6k

Some points :

  • Please use the icon 101010 to format your text as code, it is much more readable
  • Take a look at the Biopython documentation to understand how to read a fasta file (I presume you work with a fasta file)
  • Why does your file has .txt entension if it is a fasta file ?
  • You can not print a file handle like you did with print(genome) (you can... but it is not readable)

Try something like this :

from Bio import SeqIO
for record in SeqIO.parse("C:\Users\cnn\Desktop\MS(BI)\HCV.txt", "fasta"):
    printrecord.id)
    print(record.seq)

Then to go forward and recover informations about ORF, follow the link that jrj.healey gave you

ADD REPLYlink modified 29 days ago • written 29 days ago by Bastien Hervé2.3k

i already used that i want to calculate total number of genes not ORF

ADD REPLYlink written 29 days ago by anasjamshed19940

Then use a proper gene finding tools such as GeneMark or Glimmer.

Writing a python script to identify which ORFs are legitimate genes is not going to be simple.

Alternatively, use an annotation pipeline like PGAP/PROKKA, and then use biopython to count the number of gene or CDS tags that are detected in one of the annotated files.

ADD REPLYlink written 29 days ago by jrj.healey8.6k

How can i use gene annotation pipeline

ADD REPLYlink written 26 days ago by anasjamshed19940

I want to calculate genes from all 3 reading frames

ADD REPLYlink written 23 days ago by anasjamshed19940
1

calculate genes

Genes cannot be "calculated". They can be at best "found". The algorithm involved is complex, and cannot be explained over a forum discussion in my opinion.

ADD REPLYlink written 23 days ago by RamRS19k
1

We know that by now. But what you are asking for is a complex task. Not just a simple script. Please read some literature on gene prediction.

ADD REPLYlink written 23 days ago by WouterDeCoster34k

What Algorithm can i used?

ADD REPLYlink written 22 days ago by anasjamshed19940

Pick a suitable one from this list, then read their paper and replicate their algorithm.

EDIT:

Please note that your past posts on this topic have been closed because this is not a subject matter any forum can help you on - it's a project that you will need to work on (if you wish to not use the tools available already). If you continue to open questions for this topic, I'm afraid you risk a stronger response from the forum.

ADD REPLYlink modified 22 days ago • written 22 days ago by RamRS19k

Sir it is my right to ask any question on this forum until i can get the suitable answer

ADD REPLYlink written 22 days ago by anasjamshed19940

FYI, you're going to be ignored now by all of the regulars on the site, which means you will receive no further help here.

ADD REPLYlink modified 22 days ago • written 22 days ago by Devon Ryan86k

sorry sir but i can find any suitable answer of my question so thats why i am asking here again and again

ADD REPLYlink written 22 days ago by anasjamshed19940

We have already told you “suitable answers”.

If you did not get an answer you liked the first time around, why would you assume the 2nd or 3rd time would be any different?

ADD REPLYlink written 22 days ago by jrj.healey8.6k

Sir my question is can i calculate total no of genes present in HCV genome through simple python script and in the reply one person gives me a code from which orfs can be found easily but i want genes to be calculated and then some one said that use GTF file , some said use linux to calculate genes , some said use annotation pipelines and someone said its required complex algorithm But my question is that it is possible or not without algorithm?

ADD REPLYlink written 21 days ago by anasjamshed19940

It is not possible without a more complicated algorithm. This has been explained thoroughly already. Further comments from you about this will be deleted as they are not productive.

ADD REPLYlink modified 21 days ago • written 21 days ago by Devon Ryan86k

Correct, but it is not your right to ask the same question over and over again, all whilst ignoring the correct advice you have been given, because it didn’t answer your exact question (because what you want to do is not sensible).

ADD REPLYlink written 22 days ago by jrj.healey8.6k

Hello anasjamshed1994!

We believe that this post does not fit the main topic of this site.

The OPs question is beyond what is reasonably able to be assisted on the forum, and this is the second or third iteration of this same thread.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 22 days ago by jrj.healey8.6k
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