Question: find out whether cell lines are verified
1
gravatar for hibaashaban
19 months ago by
hibaashaban10
hibaashaban10 wrote:

I have a list of about 1000 cancer cell lines with associated RNA-seq expression profiles per gene. I would like to be able to confirm that these cell lines have been verified or meet a standard that can be used in research since some cell lines can be contaminated or labeled incorrectly at some point. Is there a list of "verified" cancer cell lines somewhere? Or any advice on how to go about this?

rna-seq cell-lines • 468 views
ADD COMMENTlink modified 14 months ago by Amos Bairoch40 • written 19 months ago by hibaashaban10

Just Google "list of known cancer cell lines" . https://www.mibioresearch.com/tumor-models/complete-cell-line-list/

ADD REPLYlink written 19 months ago by Arup Ghosh2.5k
3
gravatar for igor
19 months ago by
igor10k
United States
igor10k wrote:

There is a Register of Misidentified Cell Lines:

ICLAC curates a Register of cell lines that are known to be misidentified through cross-contamination or other mechanisms (e.g., mislabelling).

It seems to be actively maintained as well (the last update was less than a month ago).

ADD COMMENTlink written 19 months ago by igor10k
0
gravatar for Amos Bairoch
14 months ago by
Amos Bairoch40
Switzerland, Geneva, Swiss Institute of Bioinformatics
Amos Bairoch40 wrote:

And you can look up for your cell lines in the Cellosaurus, all those that are "problematic" are indicated and this includes all cell lines in the ICLAC register as well as other. The following FAQ explains this:

https://web.expasy.org/cgi-bin/cellosaurus/faq#Q17

Note that you can retrieve the list of cell lines in the ICLAC register by using:

https://web.expasy.org/cgi-bin/cellosaurus/search?input=%22Register%20of%20Misidentified%20Cell%20Lines%22

Here is an example in XML of the information on problematic cell lines and on the lCLAC register number:

<comment category="Registration"> International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00298 </comment>
<comment category="Problematic cell line"> Contaminated. Shown to be a HeLa derivative (PubMed=566722; PubMed=20143388). </comment>
ADD COMMENTlink written 14 months ago by Amos Bairoch40
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