I have a few samples of RNA-seq data. I have this adaptor sequience (
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG (NNNNNN= 6 nt index)). Can someone please tell me what
NNNNNNN =6 index indicates here? I was using BBMap tools to do the trimming and created the adaptor file like below, but I do not see any change in my new trimmed file (in terms of file size).