Question: Whether to include reference genome in PCA for association analysis?
gravatar for Phoenix Mu
9 months ago by
Phoenix Mu10
Phoenix Mu10 wrote:

When doing QC for GWAS, genotype from a reference panel like 1000 Genomes is typically used to calculate PCA together with our own data to find individuals with unmatched population ancestry.

My question is if I want to calculate PCs used for adjusting population admixture in my GWAS, do I also need to include a reference panel in my PCA?


pca gwas • 283 views
ADD COMMENTlink modified 9 months ago by zx87548.2k • written 9 months ago by Phoenix Mu10
gravatar for zx8754
9 months ago by
zx87548.2k wrote:

Use reference panel to get PCs, this step is useful to filter out outlier samples. Then exclude outlier samples and reference samples, and re-run PCA. These are the PCs we want to use for analysis.

See this paper for more info:

ADD COMMENTlink written 9 months ago by zx87548.2k
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