I am new to the community so please forgive any faux pas I commit.
I am looking to perform an automated cell count of in-situ hybridization labelled cells in the human cortex. More specifically, I would like to produce a density plot that communicates the pattern of labeling across the 6 cortical layers. Please find attached one of my ISH images and an example of the plot I would like to produce.
I would appreciate any information regarding what programs to use and process.
Thank you, Keon