Hi. I'm downloading some CRAM files from 1000genomes for use in variant calling. I will convert them to BAM (since most tools can't call from CRAM files). I'm a bit confused about how to go about this process since I assumed a straightforward Samtools based conversion based on a given reference FASTA.
What's the deal with the reference registry and why do I require it ?
I went through the README document on the FTP site but I'm still quite confused
I've already downloaded the hg38 FASTA from Human Genome Resources