Question: Best tool for Chimeric Transcript Analysis?
1
gravatar for esraesmeray1990
5 months ago by
Izmir Biomedicine and Genome Center
esraesmeray199010 wrote:

Hi I am searching a good tool for chimeric transcript analysis. (I have WTS) I see most of the people use TopHat2 for this aim but I also found a tool called fusioncatcher which is one of the best tool for me but it requires 24 GB RAM and my laptop has just 8GB RAM.

So if you can recommend some tools about chimeric transcript analysis, i will be appreciate.

rna-seq wts fusion chimeric ngs • 177 views
ADD COMMENTlink modified 5 months ago by guillaume.rbt600 • written 5 months ago by esraesmeray199010
1
gravatar for guillaume.rbt
5 months ago by
guillaume.rbt600
France
guillaume.rbt600 wrote:

There are a lot of fusion detection tools, you can find a 2016 non-exhaustive list in this paper : https://www.nature.com/articles/srep21597

In my opinion it is wise to test several tools, and to cross results from at least 3 tools to have relevant results, as it has be done here : https://www.sciencedirect.com/science/article/pii/S152500161830529X

ADD COMMENTlink modified 5 months ago • written 5 months ago by guillaume.rbt600

These papers are very helpful, thanks a lot.

ADD REPLYlink written 5 months ago by esraesmeray199010
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