Hi, I m running through this for single cell RNAseq data analysis. And I got this error after running a few codes. If some one could figure out that what might have I done wrong, I will appreciate. Thanks
library(R.utils) gunzip("GSE61533_HTSEQ_count_results.xls.gz", remove=FALSE, overwrite=TRUE) library(gdata) all.counts <- read.xls(’GSE61533_HTSEQ_count_results.xls’, sheet=1, header=TRUE, row.names=1) library(scater) if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("SingleCellExperiment", version = "3.8") library(SingleCellExperiment) sce <- newSCESet(countData=all.counts) Error in newSCESet(countData = all.counts) : could not find function "newSCESet"
Very strange! Check again in a fresh R-session, especially double check that scater library is loaded
Thank you Santosh.
library(scater)was loaded and still could not find function