I have PCR reaction products obtained using nematode 18S primers and sequenced on Illumina MiSeq PE 250. My aim is to identify and characterize the Nematode species from the NGS data. I do not have the reference sequence for this.
I performed BLAST on a subset of reads which does not give identity to any of the nematode species.
What existing tool/method and database should I use to perform this analysis?