Dear I am analysing a RNA sequencing data of rice samples. Sequenced were mapped using tophat and most of sample had more than 90% mapping rate (although there were 50% multimapped reads). When I used cufflink for quantification of genes, i found more than 90% genes showed fpkm value less than zero. So i am getting very less number of differentially expressed genes. What may be the reason behind this?
Question: RNA Seq analysis of rice samples
7 months ago by
alim.hcu • 10
alim.hcu • 10 wrote:
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