Dear Friends,
I have got an assembled contig of a bacteriophage. I have the annotation file from prokka and now I want to manually curate the annotation by visualizing the annotation features on the contig aligned with top 20 blast hits of the contig. Can anyone please let me know how can I do that? I tried tablet, but I don't think they have the feature to align more than one fasta file.
Right now with tablet, I can open SAM file of the reads mapped onto the contig and the reference contig fasta file. What I am looking for is to also align the 20 blast hits to the contig and then visualize the annotation. In general, a tool to manually curate the annotation.
Thanks,