Hello everyone: I am new in using DEseq2. I am trying to analyze differential expression data using a publicly available data for my final project. It consists of 3 replications of control and three replications for treatment. I am trying to compare differential gene expression between treatment and control but i am getting error message which i do not know who to fix. Here is my code. Please help me fix the problem. Codes:
countdata<-read.csv("final project.csv",header = T,row.names=1) groups<-c("control","control","control","treatment","treatment","treatment") coldata<-data.frame(row.names=colnames(countdata),groups) dds = DESeqDataSetFromMatrix(countData=countdata, colData=colData, design=~groups) dds = DESeq(dds) nrow(dds) Error mesage:> dds = DESeqDataSetFromMatrix(countData=countdata, + colData=colData, + design=~groups) Error in `rownames<-`(`*tmp*`, value = colnames(countData)) : attempt to set 'rownames' on an object with no dimensions
Thank you in advance.