Question: How to split Fastq file into Forward, reverse fastq file.
gravatar for 9521ljh
6 months ago by
9521ljh10 wrote:

Hi. i have SRR0000.sra file.

and i have SRR0000.fastq using SRA toolkit.

then i want to split it forward fastq and reverse fastq. how to????

i try to use SRA toolkit - fastq-dump -I --split-files SRR0000.sra

but the output is just the half of file. i don't think it is right for forward and reverse.

sequencing sequence genome gene • 258 views
ADD COMMENTlink written 6 months ago by 9521ljh10

this issue has been mentioned before, even here on biostars:

How to split paired end SRA file into 2 correct fastq files

in other words, have you tried looking for an answer before posting your question?

ADD REPLYlink written 6 months ago by lieven.sterck6.3k
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