Hi to all,
I've already read that Illumina reads mostly common present errors at the end.. however, never read about any issue at the beginning of them.
I have called SNVs at the beginning and end of my reads that I don't feel like trusting, although the mapping quality and phread quality appears to be of high quality.
What would you tell me? Does this look like an sequencing error?
obs: i cannot perform trimming because my library is amplicon-based Print screen of my IGV overview. Its a T>C change, it appears in both strands, but as mentioned, in the end or beginning of the amplicon