Hi all, I have a somewhat basic question.
The inputs I use for analysis are the reference genome (hg38) and my sample vcf file.
I extracted the CDS region of some gene from hg38 gff file.
After that, I extracted the consensus sequence of the cds region from my sample vcf file.
Ultimately, I want to get the amino acid sequence.
I wonder if the nucleotide sequences of the CDS region of a gene extracted above can be combined and converted into amino acid sequences.
128937678-128937818 -> GAAGTG
128936538-128936649 -> GAGGCATCTCTGA
128935037-128935044 -> GAGCGAG
128937678-128937818 -> ATCTTCGG
128936538-128936649 -> CCTTCGATG
128935591-128935700 -> TTGACAACATCT
128937678-128937818 -> AGCATTTCCTC
Combination -> GAAGTGGAGGCATCTCTGAGAGCGAGATCTTCGGCCTTCGATG TTGACAACATCTAGCATTTCCTC -> Convert to amino acid sequence
Can I get the amino acid sequence like this?