Question: Mapping Phred+64 encoded reads with Bowtie2
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gravatar for Denis
16 months ago by
Denis190
Denis190 wrote:

Hi, I faced with strange behavior of Bowtie2. I mapped reads with Phred+64 encoding by command:

bowtie2  --very-sensitive -x index -U R1.fastq -S Alignment.sam

I'm wondering why i didn't get any error message in that case? Is Bowtie2 able to determine reads encoding format automatically?

ADD COMMENTlink modified 14 months ago by Biostar ♦♦ 20 • written 16 months ago by Denis190
1

You should add --phred64 which indicates that qualities are Phred+64.

ADD REPLYlink modified 16 months ago • written 16 months ago by genomax90k

Thanks! But why i didn't get error by using this command line? I use this command a long time and only recently realized that the reads have Phred+64 encoding. How it may influence on the mapping results (everything looked well in resulted SAM file i usually got)?

ADD REPLYlink modified 16 months ago • written 16 months ago by Denis190

If I had to guess, the mapping results might be skewed as bowtie2 might (notice I said might) think that you have just really good higher quality sequences than you actually have. Not sure if bowtie2 can automatically determine the encoding of the reads or not.

ADD REPLYlink modified 14 months ago • written 14 months ago by jean.elbers1.4k
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