I am trying to learn single cell RNA-Seq data analysis. Right now I am following Seurat and Monocle documentation. I am aware of the fact that we have to create a single cell experiment object, which basically acts as a container for the scRNA data. But, I want make one these container from scratch so as to understand how the expression data and metadata are stored.
Most of the tutorials use .RDS or .MTX files to create SingleCellExperiement object. It seems like a 'black-box' for me because I cannot open and see the data as in CSV format. What I want to do is to a create CSV or TSV file for expression data and assay description and then combine these files to make a SingleCellExperiment object. Can anybody help to create a dummy SingleCellExperiment object and how metadata is added to this objects later ? Any link to the tutorials would also work.