Entering edit mode
5.3 years ago
El Niño
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10
Hi guys,
Now I have a FASTA file which is the post-assembly sequence of a bacterial genome. Now I have some pre-annotated files that stated that there are 4 insecticidal genes in my sequence. I also know that normally, these genes are located on plasmid Surprisingly, when I uploaded my sequence to PlasmidFinder, the system found only 1 gene and that does not harbor any of the 4 genes mentioned above.
Could anybody suggest better tools that I can use for plasmid detection. Best regards,
The -Anh
One I recall hearing about recently was PlasFlow. I don’t have a link/paper handy but it shouldn’t be difficult to find on google.
Never used it myself though, so can’t vouch for it.