Question: Problem with a protein analysis
0
gravatar for Estbioq
15 months ago by
Estbioq0
Estbioq0 wrote:

I have done a LC-MS/MS analysis of the proteins of a gram-positive bacterium. I need to differentiate the membrane proteins from the others, but i only have the accession number. Do you know how I can know the subcellular location of each of the thousand proteins that I have?

genome • 328 views
ADD COMMENTlink modified 15 months ago by natasha.sernova3.7k • written 15 months ago by Estbioq0

1) Look at this paper below.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582598/pdf/1471-2105-13-290.pdf

it's for multi-label proteins, but it enumerates a lot of other tools.

2)

https://www.researchgate.net/post/Is_anybody_familiar_with_subcellular_location_of_proteins

The approach above is limited to Uniprot-proteins. But you have UniProtKB accession numbers,

don't you? - it may help you.

ADD REPLYlink modified 15 months ago • written 15 months ago by natasha.sernova3.7k

Can you post a few example accession #?

ADD REPLYlink written 15 months ago by genomax91k
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