Some sequences retrieved from NCBI, contain letter 'N', which means that these nucleotide bases are not deciphered correctly, leaving an unidentified nucleotide. Should I replace N with any other base i.e. AGTC, assuming N can be any nucleotide, or I should exclude such sequences assuming that the sequencing done was not of good quality. If none of these, what I can do with such sequences in my dataset?
Question: How to handle 'N' in Nucleotide/Genes Sequences retrieved from NCBI GeneBank?
11 months ago by
ammaraakhtar3 • 0
ammaraakhtar3 • 0 wrote:
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