Question: Difference between Illumina indexes and Illumina barcode?
gravatar for Tman
4 weeks ago by
Tman0 wrote:

Is there a difference between Illumina indexes and Illumina barcodes? Some ppl. use this term synonymously and others distinguish between.

ADD COMMENTlink written 4 weeks ago by Tman0

Ultimately either term is referring to oligonucleotide sequences used to tag a fragment/sample being sequenced.

I consider Illumina P7 and P5 oligos to be tagged with index sequence. These index sequences are smaller. They are always sequenced independent of the main reads (R1/R2) in Illumina technology. Barcodes on the other hand can be custom sequences (of any length and significantly more diversity) that a bench scientist introduces into fragment being sequenced (by using custom oligos/PCR primers etc). They will generally be at the beginning (5' end) of the read so they get sequenced reliably in every fragment each time.

Some people do use these terms interchangeably but above should differentiate their practical use.

ADD REPLYlink modified 4 weeks ago • written 4 weeks ago by genomax70k

Hello Tman!

We believe that this post does not fit the main topic of this site.

Not a bioinformatics question. SeqAnswers is a better suited forum for this question. In addition, please include examples of your statements to improve your question.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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ADD REPLYlink modified 4 weeks ago • written 4 weeks ago by h.mon27k
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