Question: Creating an Object
0
gravatar for planetearth
12 months ago by
planetearth 0 wrote:

Hi, I'm having trouble with creating objects for each group. Here's what I've done so far

>group1 <- grep("old",rownames(cells@meta.data))
>group2<- grep("young",rownames(cells@meta.data))
>cells[group1]
>cells[group2]

What should I use for counts?

>group1_object <- CreateSeuratObject(counts =    , project = "group1")

Thank you.

R • 376 views
ADD COMMENTlink modified 12 months ago by RamRS28k • written 12 months ago by planetearth 0
1
gravatar for kristoffer.vittingseerup
12 months ago by
European Union
kristoffer.vittingseerup3.4k wrote:

I would suggest you start by make one Seurat object which you do joint QC and filtering on and a joint clustering (like described in this vignette). To do the sub-clustering you can simply (since you are using Seurat 3 (not Seurat2 or earlier)) use the subset() or the SplitObject() function to respectively subset or create a list with the different Seurat subsets (nice if you subsequently want to do the same analysis to all datasets). You can find example code of how both works here.

ADD COMMENTlink modified 12 months ago • written 12 months ago by kristoffer.vittingseerup3.4k
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