I'm using MISA and Primer3 in pipeline. After I got *.misa file, I put this syntax.
Then I got empty file of *.p3in. Is it normal?
By the way, I still tried to run further syntax.
primer3_core < FASTA.fa.p3in > FASTA.fa.p3out
This one showed me the description and usage of Primer3.
I put further syntax which is...
p3_out.pl FASTA.fa.p3out FASTA.fa.misa
It showed the error message like this.
Use of uninitialized value $count in concatenation (.) or string at C:\Users\admin\Documents\Work\NGS_results\MISA\p3_out.pl line 76.
Primer modelling was successful for sequences. Use of uninitialized value $count_failed in concatenation (.) or string at C:\Users\admin\Documents\Work\NGS_results\MISA\p3_out.pl line 77. Primer modelling failed for sequences.
Could anyone please help me to solve this issue?