In the reformat tool of BBmap what is the meaning of Read1_linear and Read1_log
1
0
Entering edit mode
4.6 years ago
Joseph Hughes ★ 3.0k

This is an example output from bbmap's reformat.sh tool using the following command:

 reformat.sh in1=input_R1.fq in2=input_R2.fq qhist=quality.txt

I've looked on the website and search biostars and seqanswers to try to understand what the column labels mean, with no luck.

#BaseNum    Read1_linear    Read1_log   Read2_linear    Read2_log
1   32.865  30.412  31.434  26.777
2   33.103  31.069  32.048  28.311
3   33.179  31.130  32.239  28.550
4   33.289  31.558  32.392  28.750
5   33.148  30.809  32.322  28.518

What is the meaning of Read1_linear and Read1_log? How are they calculated?

Thanks

BBmap quality reads • 1.1k views
ADD COMMENT
0
Entering edit mode

Can you post the entire command? What were you trying to do?

ADD REPLY
2
Entering edit mode
4.6 years ago
h.mon 35k

These are the per-position base quality averages, calculated with different methods: the _linear is just the average of the Phred read qualities; the _log first translate the quality score to probability, then average of the probabilities, finally translate back to Phred quality score. I think the _log should better reflect the overall base quality than the linear, specially for data sets with large variations in qualities at the same position.

ADD COMMENT

Login before adding your answer.

Traffic: 3230 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6