Bowtie 2 with remote index
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6.0 years ago
jacobcvt12 • 0

Is it possible to perform an alignment with bowtie 2 where the reference index is stored somewhere not in the file system? For example, I am thinking of several docker containers that all use the same index stored in something like AWS S3.

alignment • 1.1k views
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If they can be accessed by referring to them as a file/directory path then it should work. (https://docs.docker.com/storage/volumes/ and https://stackoverflow.com/questions/52041550/mount-s3-bucket-as-filesystem-on-aws-ecs-container )

You need to be very cautious about using cloud buckets mounted via fuse etc. They are not suitable for high performance computation. So be aware of this.

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Set up a NAS if all the containers are to be deployed in local network.

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You'll be paying for the data egress every time you use it then unless you're running the compute on AWS too (and then why not just mount the storage?).

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