Question: Can i do Mirna DEG with rna seq data ?
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gravatar for sabaghianamir70
4 months ago by
iran
sabaghianamir7010 wrote:

Hello Can i Extract the mirnas from my RNA seq data and then perform a mirna DEG ? or just i can do this with mir-seq data ?

rna-seq mirna-seq • 135 views
ADD COMMENTlink modified 4 months ago by ATpoint29k • written 4 months ago by sabaghianamir7010
1
gravatar for ATpoint
4 months ago by
ATpoint29k
Germany
ATpoint29k wrote:

No. miRNAs require special library preparation due to the short size starting from special RNA extraction methods to capture them over the actual Illumina library prep. Even if you get miRNA counts from standard RNA-seq I would not trust them.

ADD COMMENTlink modified 4 months ago • written 4 months ago by ATpoint29k
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