Question: Can i do Mirna DEG with rna seq data ?
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gravatar for sabaghianamir70
13 months ago by
iran
sabaghianamir7010 wrote:

Hello Can i Extract the mirnas from my RNA seq data and then perform a mirna DEG ? or just i can do this with mir-seq data ?

rna-seq mirna-seq • 258 views
ADD COMMENTlink modified 13 months ago by ATpoint41k • written 13 months ago by sabaghianamir7010
2
gravatar for ATpoint
13 months ago by
ATpoint41k
Germany
ATpoint41k wrote:

No. miRNAs require special library preparation due to the short size starting from special RNA extraction methods to capture them over the actual Illumina library prep. Even if you get miRNA counts from standard RNA-seq I would not trust them.

ADD COMMENTlink modified 13 months ago • written 13 months ago by ATpoint41k
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