"Kallisto" to map RNASeq data to
reference transcriptome and have done my analysis.
How I have a confusing outcome. Out of my 5
T-DNA knockout genotypes, two of them are showing the expressing of the gene (even higher than the WT).
These are published lines and I have already genotyped them by PCR before.
Is this because of the
How can I check this? Is there any way of mapping?
P.S. I do not believe there is cross-contamination!
I am not a bioinformatician and would appreciate it if there is a step by step detailed protocol to do this.