Hi, I have a single-end 16S Sanger sequencing generated using two primers (27F and 785F). I want to identify bacterial strain using this data. How to analyse this data? My initial attempts, I tried to merge read from two primers but there are no overlapping sequences. So, 1. Should I take a union of these two reads and do search to get hits? or 2. Individually search using each read and see what are the hits? Thank you.
Question: 16S Sanger single end read assembly
13 months ago by
nilus1432 • 30
nilus1432 • 30 wrote:
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