Downloading RNA-Seq Dataset

Question

[naseerkhan861]

I am trying to download RNA-Seq data from This link. I have downloaded and Installed IBM aspera connect for windows but still it is not working as I donot know how to load downloading links in aspera connect in windows 10. Does somebody know the process to download SRA files fastly using aspera connect or any other tool in windows 10?

Regards

Answer 1

[ATpoint]

You will have to use prefetch from the SRAtoolkit which uses Aspera internally (see the documentation on how to set it up) followed by fastq-dump to get the SRA files / convert them to fastq. Still, it would be way easier with a Linux partition and Fast download of FASTQ files from the European Nucleotide Archive (ENA) as I already suggested in a previous thread.

[mathavanbioinfo]

Hi, You can download fastq file from ENA database using wget Linux command . One simple step enter your accession number in the text box in ENA database. Under the fastq title right click it copy the link address and paste the link Linux terminal as wget "link address". Then you will get file