Question: Checking quality of predesigned probes for multiplex qPCR
0
gravatar for wijexex511
6 months ago by
wijexex5110
wijexex5110 wrote:

Hello, we have a single common primer and a set of specific probes for a set of sequences (over 50). How can we check quality of this design, can you recommend us some software and remind us the steps we should definitely follow ? Thanks in advance

multiplex qpcr • 129 views
ADD COMMENTlink modified 6 months ago by trausch1.5k • written 6 months ago by wijexex5110

Another option: https://www.drive5.com/usearch/manual/cmd_search_oligodb.html

Or make a multiple sequence alignment

ADD REPLYlink written 6 months ago by gb1.8k
0
gravatar for trausch
6 months ago by
trausch1.5k
Germany
trausch1.5k wrote:

I am not sure I fully understand your question. If you want all primer binding sites and possible amplicons across a genome for your multiplexed qPCR design then this tool should work: https://www.gear-genomics.com/silica/

ADD COMMENTlink written 6 months ago by trausch1.5k
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