Question: UMI on the fastq header
0
gravatar for Ati
5 days ago by
Ati30
Ati30 wrote:

I have a paired-end bulk RNAseq data. An example of a read header in my data is: @A00379:151:HW2HVDSXX:4:1101:21748:1078_CGCTA 3:N:0:CAGTTCTG+TGACTGAC Based on @Instrument:RunID:FlowCellID:Lane:Tile:X:Y:UMI ReadNum:FilterFlag:0:IndexSequence or SampleNumber, CGCTA is the UMI. But what is the numbers before it 1078_? Is this the read ID?

ADD COMMENTlink modified 5 days ago • written 5 days ago by Ati30
2

It should be the Y co-ordinate of the particular cluster. In this case a _ has been used to append the UMI.

Read ID should be this entire string: @A00379:151:HW2HVDSXX:4:1101:21748:1078

ADD REPLYlink modified 5 days ago • written 5 days ago by genomax75k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 858 users visited in the last hour