Question: UMI on the fastq header
gravatar for Ati
9 months ago by
Ati30 wrote:

I have a paired-end bulk RNAseq data. An example of a read header in my data is: @A00379:151:HW2HVDSXX:4:1101:21748:1078_CGCTA 3:N:0:CAGTTCTG+TGACTGAC Based on @Instrument:RunID:FlowCellID:Lane:Tile:X:Y:UMI ReadNum:FilterFlag:0:IndexSequence or SampleNumber, CGCTA is the UMI. But what is the numbers before it 1078_? Is this the read ID?

ADD COMMENTlink modified 9 months ago • written 9 months ago by Ati30

It should be the Y co-ordinate of the particular cluster. In this case a _ has been used to append the UMI.

Read ID should be this entire string: @A00379:151:HW2HVDSXX:4:1101:21748:1078

ADD REPLYlink modified 9 months ago • written 9 months ago by genomax90k
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