Question: How to produce a pdb file after introducing multiple point mutations?
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gravatar for karthikqk95
5 weeks ago by
karthikqk950 wrote:

i have introduced a mutations in an protein sequence based on literature review. i have their FASTA files, i have tried mutagenesis tool in pymol to produce multiple point mutations, then i used the same file for GROMACS molecular dynamics simulations to study its stability i am facing errors, i have tried SCWRL4 also, that output file also didnot work, Give me suggestion, like How to produce a pdb file after introducing multiple point mutations?

ADD COMMENTlink written 5 weeks ago by karthikqk950

Is this a protein with a known structure already?

Also, please put more effort in to your question...

i am facing errors

...and...

also didnot work

...are not useful bits of information to us.

ADD REPLYlink modified 5 weeks ago • written 5 weeks ago by Joe16k
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