Question: How to construct draft genome of virus using Trinity de novo assembler?
gravatar for mathavanbioinfo
4 months ago by
mathavanbioinfo50 wrote:

Hi, I am working on the viral genome ( Single negative-stranded RNA virus) and the reads generated are from the ion torrent ( Amplicons) method. After performing de novo assembly using trinity tool produced contigs are in the form of a single fasta file. The next step is to construct a draft genome from the contigs. Is there any tools/ software to create a draft genome from the contigs. Kindly recommend your advice and suggestions and suitable methodology Response highly appreciated Thank you

amplicons trintiy rna virus • 170 views
ADD COMMENTlink written 4 months ago by mathavanbioinfo50

I think trinity is denovo RNAseq assembler. Try spades.

ADD REPLYlink written 4 months ago by cpad011213k
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