A mirtron on my small RNA-seq data

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4.2 years ago

yronimuz • 0

Hello,

As I have finished the differential expression analysis of my small RNA-seq data, I found on mirtron on my DE miRNAs. And it is only specific to the Sus scrofa species and not annotated in humans. Is this normal to find a mirtron on my small RNA-seq data or maybe is this just a false positive? I've used DeSeq2 to analyze my data.

Thank you.

mirna rna RNA-Seq genome next-gen • 746 views

ADD COMMENT • link updated 4.2 years ago by Biostar 20 • written 4.2 years ago by yronimuz • 0

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Why not? Obviously you'll have to verify its expression in the lab

ADD REPLY • link 4.2 years ago by Asaf 10k

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Thank you for the replies and very funny with the megatrons haha.

One more question, if this is not annotated in other species, do you have any strategies on how can I predict its target genes as well? Since on miRBase, it does not have much mention on papers - actually just one.

ADD REPLY • link 4.2 years ago by yronimuz • 0

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For those who are not familiar with mirtrons.

ADD REPLY • link 4.2 years ago by GenoMax 141k

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Not to be confused with megatrons

ADD REPLY • link 4.2 years ago by Asaf 10k

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I actually thought it refers to all miRNAs that reside in introns, including the ones transcribed with their own promoter and going through the traditional DROSHA-dependent splicing. How can you tell it's DROSHA-independent from sRNA-seq?

ADD REPLY • link 4.2 years ago by Asaf 10k

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