RNA velocity arrows means

Question

[alpha09]

I want to know how arrows are generated in RNA velocity? I read papers but I didn't get clear view of it.

Second thing, can I apply RNA Velocity on the data that has no time points? I have data on adult stage of a species.

Thanks in advance.

Answer 1

[Kevin Blighe]

The arrows point to the predicted future state of their respective cells.

Second thing, can I apply RNA Velocity on the data that has no time points? I have data on adult stage of a species.

Not necessarily - please take a look here: https://jef.works/blog/2020/01/14/rna_velocity_analysis_tutorial_tips/

Kevin