Which format to download ESTs for annotation with MAKER?

Question

[mrmrwinter]

Which format do i need to download ESTs in from GenBank?

Do i need the Complete Features? Or just the Sequence?

Or even just the gene information?

This is to run into MAKER so needs to be in FASTA

Many thanks.

Answer 1

[Juke34]

As you said it needs to be in Fasta format.

Here a complete programmatic solution to get all EST related to your species using conda and the GAAS toolkit.

conda create -n gaas  
conda activate gaas  
conda install gaas
gaas_ncbi_get_reference_data.pl --db nucest --organism species_name

Answer 2

[lieven.sterck]

As far as I know it has to be the fasta format (== the sequences them self thus)

select "format as FASTA" from the top left and then sent to file ( upper right)