Hi all,
I have a RNAseq experiment to measure gene expression with 3 treatment and 3 control, but one of my treatment failed, now I just have 2 treatment vs 3 control. My question is that can I take another treatment sequenced in single end read to replace for one of my failed treatment? I am just beginner and have no experience in this area. I am much appreciated for your help.
The question is will it add noise or decrease noise. I think that the chance that a gene will be different between the two batches is higher than having a noisy reading in the other two treatments. So yeah, do it 2 vs 3, not too bad, depends on the experiment though.
Thank you very much Asaf, I forgot to mention that my 3 treatments and 3 controls are in pair end reading. Could you please don't mind explain how it depends on the experiment? Thanks a lot.
What Devon said.
Thanks a lot Asaf, Cheers,