Entering edit mode
4.1 years ago
rasoulfarzaneh
•
0
Hi The script I have is
tophat -p 4 --b2-very-sensitive -G '/home/rasoul/Desktop/Data/ngs/genome/annotation.gtf' /Home/rasoul/Desktop/Data/ngs/genome/genome '/Home/rasoul/Desktop/Data/ngs/SRR941816/keep16.gz'
Error
[2020-04-05 18:15:48] Beginning TopHat run (v2.1.1)
-----------------------------------------------
[2020-04-05 18:15:48] Checking for Bowtie
Bowtie version: 2.2.5.0
[2020-04-05 18:15:48] Checking for Bowtie index files (genome)..
Error: Could not find Bowtie 2 index files (/Home/rasoul/Desktop/Data/ngs/genome/genome.*.bt2l)
If you are trying to run a
transcriptome
only run with-G
option then you are not supposed to provide reads for that run.It also looks like you need to use
/Home/rasoul/Desktop/Data/ngs/genome/
as the index base.You should also consider switching to a newer aligner than TopHat.