Online pipeline for MSA and NJ tree generation bootstrap supported
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Entering edit mode
12 months ago
lessismore ★ 1.1k

Dear all,

can somebody recommend a simple and solid online pipeline for multiple sequence alignment (MSA) and tree generation (NJ) of about 1K protein sequences ? I've found https://www.ebi.ac.uk/Tools/msa/clustalo/ which seems to cover this goal but its not really clear to me how does it produce the nexus file for visualizing the tree + it shows only the branch length while i'm actually searching for some bootstrap analyses.

thanks in advance

MSA NJ phylogeny • 594 views
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Entering edit mode
12 months ago

Hi,

I highly recommend on-line service Phylogeny.fr. It performs:

  • Multiple sequence alignment (Clustal / MUSCLE / T-Coffee / ProbCons)
  • MSA curation (Gblock / remove positions with gaps)
  • Tree construction with bootstrap (NJ / Parsimony / Maximum Likelihood / Bayes)
  • Tree visualization
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