Entering edit mode
4.0 years ago
Bioinfo
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Hello Everyone , i hope you're doing well
i'm using Gap Filler , after scaffolding , in LIbrary file i specefied Bowtie for mapping , but it didn't work i ve got this error message :
ITERATION 1:
=>Sat Apr 18 18:10:40 2020: Mapping reads to scaffolds, reading bowtie output and storing unmapped reads
Warning: Empty input file
Reference file does not seem to be a FASTA file
i don't know what went wrong , my reference File seems to be in Fasta file
Please post the full command line and a
head
of your problematic file. This is standard for debugging questions, please do it as default in the future.Thank you for your reply heres the command i used :
GapFiller.pl -l Gap_Filler_Library -s file.fasta -m 30 -b GapFiller_RESULTS -t 4
and here's the lubrary file
Lib1 bowtie R_1_Miseq.fastq R_2_Miseq.fastq 400 0.25 FR
and here's the header of the scaffold file
That is not a fasta file. A fasta is like
I am not into assembly myself but the tool apparently wants a fasta file from your previous assembly the contains the sequences of the scaffolds. Is this available to you?
yes it's like you said
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